Analysis of androgen-5&-reductase by an enzyme kinetic method

an infinite planar array of rectangular microstrip patch antenna analysis

the methods which are used to analyze microstrip antennas, are divited into three categories: empirical methods, semi-empirical methods and full-wave analysis. empirical and semi-empirical methods are generally based on some fundamental simplifying assumptions about quality of surface current distribution and substrate thickness. thses simplificatioms cause low accuracy in field evaluation. ful...

A new "kinetic" method for enzyme analysis suitable for automation.

A method is proposed for automatic instrumentation of enzyme activity determinations which involve spectrophotometric measurements. The procedure utilizes a kinetic method to produce one instrumental reading that is proportional to enzyme concentration. The single spectrophotometric reading can be processed by analog or digital computation, using the known absorptivity constant of the reactant ...

An incremental isoconversional method for kinetic analysis

Correlation between Cytochrome P450, 5-alpha Reductase, and Androgen Receptor Levels in Patients with Type 2 Diabetes Mellitus

Objective: Type 2 diabetes mellitus (T2DM) is one of the most common chronic diseases. The CYP450 plays an important role in the biosynthesis of steroid hormones and the hormonal activity is mediated by the androgen receptor (AR) and the enzyme 5-alpha reductase (5αR). Therefore, this study aimed to investigate the relationship between these factors in T2DM. Materials and Methods: This case-co...

Development of a Sensitive Spectrofluorometric-Multivariate Calibration Method for Enzyme Kinetic of Aldehyde Oxidase

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...